The tools, discovered in a cave named Lapa do Picareiro, located near the Atlantic coast of central Portugal, link the site with similar finds from across Eurasia to the Russian plain. The discovery supports a rapid westward dispersal of modern humans across Eurasia within a few thousand years of their first appearance in southeastern Europe. The tools document the presence of modern humans in westernmost Europe at a time when Neanderthals previously were thought to be present in the region. The finding has important ramifications for understanding the possible interaction between the two human groups and the ultimate disappearance of the Neanderthals.

"The question whether the last surviving Neanderthals in Europe have been replaced or assimilated by incoming modern humans is a long-standing, unsolved issue in paleoanthropology," said Lukas Friedl, an anthropologist at the University of West Bohemia in Pilsen, Czech Republic, and project co-leader. "The early dates for Aurignacian stone tools at Picareiro likely rule out the possibility that modern humans arrived into the land long devoid of Neanderthals, and that by itself is exciting."

Until now, the oldest evidence for modern humans south of the Ebro River in Spain came from Bajondillo, a cave site on the southern coast. The discovery of stone stools characterized as Aurignacian, technology associated with early modern humans in Europe, in a secure stratigraphic context at Picareiro provide definitive evidence of early modern human arrival.

"Bajondillo offered tantalizing but controversial evidence that modern humans were in the area earlier than we thought," Haws said. "The evidence in our report definitely supports the Bajondillo implications for an early modern human arrival, but it's still not clear how they got here. People likely migrated along east-west flowing rivers in the interior, but a coastal route is still possible."

"The spread of anatomically modern humans across Europe many thousands of years ago is central to our understanding of where we came from as a now-global species," said John Yellen, program director for archaeology and archaeometry at the National Science Foundation, which supported the work. "This discovery offers significant new evidence that will help shape future research investigating when and where anatomically modern humans arrived in Europe and what interactions they may have had with Neanderthals."

The Picareiro cave has been under excavation for 25 years and has produced a record of human occupation over the last 50,000 years. An international research team from the Interdisciplinary Center for Archaeology and Evolution of Human Behavior (ICArEHB) in Faro, Portugal, is investigating the arrival of modern humans and extinction of Neanderthals in the region.

The project is led by Haws, Michael Benedetti of the University of North Carolina Wilmington, and Friedl, in collaboration with Nuno Bicho and João Cascalheira of the University of Algarve, where ICArEHB is housed, and Telmo Pereira of the Autonomous University of Lisbon.

With support from U.S. National Science Foundation grants to Haws and Benedetti, the team has uncovered rich archaeological deposits that include stone tools in association with thousands of animal bones from hunting, butchery and cooking activities.

Sahra Talamo of the University of Bologna, Italy, and the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, joined the research team to determine the age of the early modern human and Neanderthal occupations. She used state-of-the-art bone pretreatment and accelerator mass spectrometry (AMS) to date the bones that show evidence of butchery cut marks and intentional breakage by humans to extract bone marrow, a highly prized and nutritious food consumed by ancient people. The dating results place the modern human arrival to the interval between 41,000 and 38,000 years ago. The last Neanderthal occupation at the site took place between 45,000 and 42,000 years ago.

"The radiocarbon results from Lapa do Picareiro are not only very precise in terms of the dating method, but also demonstrate the meticulous work of the archeologists at the site," Talamo said. "The importance of collaboration between the radiocarbon specialist and the archaeologists is essential in order to obtain an accurate chronology like in the case of Picareiro."

Spatial analysis of high-resolution three-dimensional data confirmed the precise stratigraphic relationships between artifacts and radiocarbon samples and revealed discrete layers of occupation at the site.

"Analysis of high-resolution spatial data is crucial for documenting and observing lenses of human occupation and reconstructing occupational patterns, especially in cave environments where complex formation processes exist," said Grace Ellis, a Ph.D. student at Colorado State University studying landscape archaeology and ancient settlement patterns.

This was backed up by artifact refitting that showed the stone tools were not moved through post-depositional processes.

"Refitting is a task that requires a lot of time and patience, and in this case, it really was worthwhile because the results verified the geospatial observations," said Pereira, an archaeologist who specializes in stone technology.

While the dates suggest that modern humans arrived after Neanderthals disappeared, a nearby cave, Oliveira, has evidence for Neanderthals' survival until 37,000 years ago. The two groups may have overlapped for several thousand years in the area.

"If the two groups overlapped for some time in the highlands of Atlantic Portugal, they may have maintained contacts between each other and exchanged not only technology and tools, but also mates. This could possibly explain why many Europeans have Neanderthal genes," said Bicho, director of ICArEHB.

"Besides genetic and archeological evidence, high-resolution temporal context and fossil evidence across the continent is crucial for answering this question. With the preserved key layers dated to the transitional period, we are now awaiting human fossils to tell us more about the nature of the transition," Friedl said.

Despite the overlap in dates, there does not appear to be any evidence for direct contact between Neanderthals and modern humans. Neanderthals continued to use the same stone tools they had before modern humans arrived, bringing a completely different stone technology.

"Differences between the stone tool assemblages dated before and after about 41,000 years ago are striking at Picareiro," said Cascalheira, an ICArEHB board member and specialist on stone tool technology. "Older levels are dominated by quartzite and quartz raw materials and marked by the presence of Levallois technology, a typical element of Neanderthal occupations in Europe. Aurignacian levels, on the other hand, are dominated by flint and the production of very small blades that were likely used as inserts in arrow shafts for hunting."

Flint also was used to make tools for butchering animals such as red deer, ibex and possibly rabbits. The team recovered a few red deer canine teeth, often used as personal adornments, but so far these do not show traces of manufacturing jewelry.

"The bones from Lapa do Picareiro make up one of the largest Paleolithic assemblages in Portugal, and the preservation of these animal bones is remarkable," said Milena Carvalho, a Ph.D. candidate at the University of New Mexico and ICArEHB researcher studying the diets and paleoecology of Neanderthals and modern humans. "The collection will provide tremendous amounts of information on human behavior and paleoecology during the Paleolithic and we will be studying it for decades."

The cave sediments also contain a well-preserved paleoclimatic record that helps reconstruct environmental conditions at the time of the last Neanderthals and arrival of modern humans.

"We studied changes in the size of limestone clasts and the chemistry of muddy fine sediment filling the cave to understand the paleoclimatic context for the transition," Benedetti said. "Our analysis shows that the arrival of modern humans corresponds with, or slightly predates, a bitterly cold and extremely dry phase. Harsh environmental conditions during this period posed challenges that both modern human and Neanderthal populations had to contend with."

The cave itself has an enormous amount of sediment remaining for future work and the excavation still hasn't reached the bottom.

"I've been excavating at Picareiro for 25 years and just when you start to think it might be done giving up its secrets, a new surprise gets unearthed," Haws said. "Every few years something remarkable turns up and we keep digging."

Story Source:

Materials provided by University of Louisville. Original written by Betty Coffman. Note: Content may be edited for style and length.

Related Multimedia:

• View of the excavation site in Lapa do Picareiro

Journal Reference:

1. Jonathan A. Haws, Michael M. Benedetti, Sahra Talamo, Nuno Bicho, João Cascalheira, M. Grace Ellis, Milena M. Carvalho, Lukas Friedl, Telmo Pereira, Brandon K. Zinsious. The early Aurignacian dispersal of modern humans into westernmost Eurasia. Proceedings of the National Academy of Sciences, 2020; 202016062 DOI: 10.1073/pnas.2016062117